rabbit anti-cd11b  (Novus Biologicals)

Journal: Journal of Lipid Research

Article Title: Hepatoprotective drug screening identifies daclatasvir, a promising therapeutic candidate for MASLD by targeting PLIN2

doi: 10.1016/j.jlr.2025.100835

Figure Lengend Snippet: Daclatasvir treatment ameliorates liver damage and fibrosis in MCD diet-induced MASH mouse model. A: Schematic illustration of the experimental workflow for the daclatasvir treatment strategy in a methionine-choline deficient (MCD) diet-induced MASH mouse model. B: Liver weight, LW/BW, and LW/BMI were recorded for MCD diet-fed mice after 3 weeks of treatment with either vehicle or daclatasvir. C: Representative images of H&E, PSR, and CD11b staining of liver samples from vehicle or daclatasvir treated mice at 4 weeks of MCD diet administration. Scale bar, 200 μm for H&E and PSR; 100 μm for CD11b. D: Quantitative results for PSR and CD11b staining shown in (C). E: Enzyme activities of serum hepatic transaminases in MCD diet-fed mice after 3 weeks of treatment with either vehicle or daclatasvir. F–H: The mRNA expression of genes associated with lipid catabolism (F), inflammation (G), and fibrosis (H) in liver tissues from MCD diet-induced MASH mice following 3 weeks of daclatasvir or vehicle treatment. For (B–H), n = 6 mice per group. Data are presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01; Student’s t test for statistics.

Article Snippet: The sections were incubated with a rabbit anti-CD11b primary antibody (BM3925, Boster) overnight at 4°C, followed by incubation with a goat anti-rabbit fluorophore-conjugated secondary antibody (A-11036, Invitrogen).

Techniques: Staining, Expressing

Journal: Frontiers in Oncology

Article Title: The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice

doi: 10.3389/fonc.2025.1603293

Figure Lengend Snippet: Immune cells infiltration of pancreatic tissue with PC (Kras/Cre mice) and control (Cre mice) after Sal or CER injections, (A) representative images of CD20 + , CD3 + , CD4 + , CD8 + cells staining; scale bars represent 50 µm, (B) median number of CD20 + , CD3 + , CD4 + , and CD8 + cells, (C) representative images of staining CD11b + cells (scale bars represent 50 µm) and percentage of mice with CD11b + cells staining, scale bars represent 50 µm, n = 5 mice/group. Kras/Cre, mice with Kras mutation; Cre, mice without Kras mutation; Sal, saline; CER, cerulein; hpfs, high power fields. Statistically significant differences (p value < 0.05) between mice are marked * (for details see the text).

Article Snippet: In the next stage, the slides were incubated with rabbit anti-mouse antibodies CD11b (Biorbyt, Catalog Number: orb31554, dilution 1:100), CD20 (Biorbyt, Catalog Number: orb374711, dilution 1:200), CD3 (Biorbyt, Catalog Number: orb348965, dilution 1:100), CD4 (Biorbyt, Catalog Number: orb4830, dilution 1:100) and CD8 (Biorbyt, Catalog Number: orb323288, dilution 1:50) for 30 minutes at room temperature.

Techniques: Control, Staining, Mutagenesis, Saline

Journal: Frontiers in Oncology

Article Title: The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice

doi: 10.3389/fonc.2025.1603293

Figure Lengend Snippet: Immune cells infiltration of pancreatic tissue with PC (Kras/Cre mice) and control (Cre ctr mice) after FMT and sham treatments, (A) representative images of staining CD20 + , CD3 + , CD4 + , CD8 + cells, scale bars represent 50 µm, (B) median number of CD20 + , CD3 + , CD4 + , CD8 + cells; (C) representative images of staining CD11b + cells (scale bars represent 50 µm) and percentage of mice with CD11b + cells staining estimeted as a weak (≤ 5 positive cells) or strong (>5 positive cells in site of view).; Kras/Cre, mice with Kras mutation; Cre, mice without Kras mutation; FMT, fecal microbiota transplantation; n = 5 mice/group; Statistically significant differences (p value < 0.05) between mice are marked * (for details see the text).

Article Snippet: In the next stage, the slides were incubated with rabbit anti-mouse antibodies CD11b (Biorbyt, Catalog Number: orb31554, dilution 1:100), CD20 (Biorbyt, Catalog Number: orb374711, dilution 1:200), CD3 (Biorbyt, Catalog Number: orb348965, dilution 1:100), CD4 (Biorbyt, Catalog Number: orb4830, dilution 1:100) and CD8 (Biorbyt, Catalog Number: orb323288, dilution 1:50) for 30 minutes at room temperature.

Techniques: Control, Staining, Mutagenesis, Transplantation Assay